[英文释义] A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome,encoding the same mutant phenotype,is introduced into that cell.
By using the complementation test an unknown mutant can be rapidly assigned to a particular gene . 采用互补测验可迅速地将某一未知突变型归属于某一特定基因。
Human elongator complex serves a role in chromatin remodeling through modifying the lysine residues of histone h3 and h4 during transcription elongation in higher cells . the main results and conclusions are as followings . 1 using depletion strain of yeast as material, we have established a system for complementation test and gene expression analysis of human elongator complex 4、利用点突变和plasmidshuffle等技术构建了含h3k14一r、h4ks~r单突变和h3k14一侧h4ks一r双突变的e1p3乙菌株,通过功能互补实验分析少凡p3对突变株的补偿功能,证实酵母elongator可对h314k/h4sk进行乙酞化修饰。